Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins.
It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function.
Diphtheria toxin inhibits protein synthesis in eukaryotic cells by catalyzing inactivation of elongation factor-2 (EF2).
Entry of toxin into cells is dependent on binding to the cell surface.
Mice and rats are resistant to diphtheria toxin, and the resistance extends to cultured cells.
Mouse-human hybrid cells are sensitive to diphtheria toxin.
Creagan et al.
(1975) showed that sensitivity is determined by human chromosome 5, which presumably carries a gene for a receptor for toxin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse HBEGF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser25-Thr160 of mouse HBEGF, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
