Beta globin (HBB, beta-globin) is a protein that, along with alpha globin (HBA), makes up the most common form of hemoglobin in adult humans.
The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains.
The gene is located in the beta-globin locus.
Expression of beta globin and the neighboring globins in the beta-globin locus is controlled by single locus control region (LCR).
The order of the genes in the beta-globin cluster is 5' - epsilon – gamma-G – gamma-A – delta – beta - 3'.
Mutant beta globin is responsible for the sickling of red blood cells seen in sickle cell anemia.
Absence of beta chain causes beta-zero-thalassemia.
Reduced amounts of detectable beta globin causes beta-plus-thalassemia.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes equine HBb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Eq
Immunogen：Recombinant protein corresponding to Met1-His147 of equine HBb, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
