Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
The octamer wraps approximately 147 bp of DNA.Core component of nucleosome.
Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes H4. Species Crossreactivity: human, mouse, rat, porcine, monkey, feline, canine, guinea pig, bovine, equine, chicken
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo Ca Ch Eq Fe Gp Hu Mk Mo Po Rt
Immunogen：Recombinant protein corresponding to Ser2-Gly103 of H4, fused to His-Tag at N-terminal expressed in E. coli.
