Glycophorin C (GPC) is a single polypeptide chain of 128aa and is encoded by a gene on the long arm of Chromosome 2 (2q14-q21).
Two isoforms are known and the gene is expressed in a wide variety of tissues including kidney, thymus, stomach, breast, adult liver and erythrocyte.
In the non erythroid cell lines, expression is lower than in the erythrocyte and the protein is differentially glycosylated.
In the erythrocyte glycophorin C makes up ~4% of the membrane sialoglycoproteins.
The GPC gene is organized in four exons distributed over 13.5 kilobase pairs of DNA and contains two directly repeated domains 3.4 kilobase pairs long which may be derived from a recent duplication of a single ancestral domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GYPC.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Ile95 of mouse GYPC, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
