Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds.
Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta
The theta class includes GSTT1 and GSTT2.
The GSTT1 and GSTT2 share 55%aa sequence identity and both of them were claimed to have an important role in human carcinogenesis.
The GSTT1 gene is located approximately 50kb away from the GSTT2 gene.
The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GSTt1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Leu7-Leu231 of rat GSTt1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
