By searching a genomic sequence database with the GSTA1 and GSTA4 sequences, Morel et al.
(2002) identified 1 BAC and 3 PAC clones covering more than 400 kb and containing the entire GST-alpha gene cluster.
The cluster consists of 5 genes, GSTA1, GSTA2, GSTA3, GSTA4, and GSTA5, as well as 7 pseudogenes.
The deduced GSTA3 protein contains 222 amino acids.
By RT-PCR using gene-specific probes, Morel et al.
(2002) demonstrated that GSTA1, GSTA2, and GSTA4 transcripts are widely expressed in human tissues, whereas the GSTA3 transcript appears to be a rare message subject to splicing defects.
Although GSTA5 appears to be a functional gene, expression of GSTA5 could not be detected in any tissues examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes chicken GSTa3.
Isotype：IgY
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Ch
Immunogen：Recombinant protein corresponding to chicken GSTa3, Met1-His229.
