Gelsolin is an actin-regulatory protein that modulates actin assembly and disassembly, and is believed to regulate cell motility in vivo through modulation of the actin network.
In addition to its actin-regulatory function, gelsolin has also been proposed to affect cell growth.It is first isolated from rabbit lung macrophages as a modulator of the cytoplasmic actin gel−sol transformation.
Gelsolin can sever actin filaments and cap the fast-growing ends of the filaments in vitro; this promotes actin disassembly.
Gelsolin also has the ability to nucleate actin polymerization.
These functions are activated by Ca2 and inhibited by polyphosphoinositides (PPIs).
Such observations have led to the view that gelsolin regulates actin reorganization in response to changes in the concentrations of Ca2 and PPIs in living cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GS.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg577-Asp771 of human Gelsolin, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
