The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties.
Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C.
Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8.
Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Alternative splice variants of the GRM1 gene have been described but their full-length nature has not been determined.
Mice lacking functional glutamate receptor 1 were reported in 1994.
By homologous recombination mediated gene targeting those mice became deficient in mGlu receptor 1 protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GRM1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser165-Glu592 of human GRM1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
