G protein-coupled receptor kinase 4 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation.
This gene has been linked to both genetic and acquired hypertension.
G protein-coupled receptor kinases are a family of serine/threonine kinases that appear to play a role in receptor desensitization.
Beta-adrenergic receptor kinase-1 and rhodopsin kinase are examples.
They phosphorylate the activated form of the corresponding receptor, increasing its ability to interact with beta-arrestin or retinal arrestin, respectively.
Binding of the arrestins to the receptors then blocks further activation of the respective signal transduction pathways.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GRK4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe187-Phe449 of human GRK4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
