Growth factor receptor-bound protein 10 is a protein belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules.
This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors.
Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression.
This gene is imprinted in a highly isoform- and tissue-specific manner.
GRB10 gene is alternatively spliced, producing transcripts that encode proteins either with or without a 46aa stretch that contains part of the PH domain.
The SH2 and PH domains of the human GRB10 protein are 99% and 84% identical, respectively, to those of mouse Grb10.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human Grb10.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Thr392-Leu594 of human Grb10, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
