Glutathione reductase, also known as GSR or GR, is an enzyme (EC 1.8.1.7) that reduces glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant.
For every mole of oxidized glutathione (GSSG), one mole of NADPH is required to reduce GSSG to GSH.
The enzyme forms a FAD bound homodimer.
The glutathione reductase is conserved between all kingdoms.
In bacteria, yeasts, and animals, one glutathione reductase gene is found; however, in plant genomes, two GR genes are encoded.
Drosophila and Trypanosomes do not have any GR at all.
In these organisms, glutathione reduction is performed by either the thioredoxin or the trypanothione system, respectively.
Members of the glucose transporter (GLUT) family, including SLC2A14, are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells.
GLUTs show tissue and cell-type specific expression.
By database analysis, followed by PCR of a human testis cDNA library, Wu and Freeze (2002) cloned SLC2A14.
They identified 2 alternatively spliced forms.
The short isoform has 10 exons and encodes a 497aa protein that shares 94.5% identity with SLC2A3.
The long isoform has 11 exons and encodes a 520aa protein that differs from the short isoform by inclusion of exon 1b.
SLC2A14 contains 12 transmembrane domains, a putative glycosylation site, and sugar transporter signature motifs.
RT-PCR analysis showed that both isoforms were expressed in testis only.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro7-Leu246 of rat Glutathione Reductase, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
