Cartilage glycoprotein-39is one of the major secreted proteins of ex vivo cultured human articular chondrocytes, synovial cells, and macrophages and also expressed by mature neutrophils (from myelocyte-metamyelocyte stage).
High levels of YKL-40 are found in synovial fluid from patients with active rheumatoid arthritis (RA) and in the hippocampus of schizophrenic patients.
YKL-40 belongs to the 18-glycosyl hydrolase (mammalian chitinase) family, and is one of the putative autoantigens in rheumatoid arthritis (RA).
High levels of YKL-40 are found in synovial fluid from patients with active RA.
The name 'YKL' stands for its N-terminal tripeptide sequence, and unlike the other member YKL39 which is unglycosylated, YKL40 is glycosylated.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GP39.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Tyr117-Lys364 of rat GP39, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
