The enzyme and zymogen forms of digestive enzymes are stored in secretory zymogen granules (ZGs) in the exocrine pancreas.
Since peptide hormones and cholinergic agents enhance release of ZG contents into the apical medium, ZGs constitute an important storage compartment in the regulated secretory pathway.
The process of zymogen storage captures large quantities of cellular membranes destined for targeting to the apical plasma membrane
Fukuoka et al.
(1991) isolated dog cDNAs encoding GP2, a 75kD protein that represents the major protein in ZG membranes.
They found that GP2 is a GPI (glycosylphosphatidylinositol)-linked membrane protein released from the membrane of mature zymogen granules by an enzymatic mechanism.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Phe307-Asn512 of human GP2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
