The enzyme and zymogen forms of digestive enzymes are stored in secretory zymogen granules (ZGs) in the exocrine pancreas.
Since peptide hormones and cholinergic agents enhance release of ZG contents into the apical medium, ZGs constitute an important storage compartment in the regulated secretory pathway.
The process of zymogen storage captures large quantities of cellular membranes destined for targeting to the apical plasma membrane
Fukuoka et al.
(1991) isolated dog cDNAs encoding GP2, a 75kD protein that represents the major protein in ZG membranes.
They found that GP2 is a GPI (glycosylphosphatidylinositol)-linked membrane protein released from the membrane of mature zymogen granules by an enzymatic mechanism.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-3ug/mlImmunohistochemistry (Paraffin): 5-30ug/mlImmunocytochemistry: 5-30ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa301-506 of mouse GP2, expressed in E. coli.
