84-4419-37　GP2 (Glycoprotein 2, Zymogen Granule Membrane , ZAP75,Pancreatic Secretory Granule Membrane Major Glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2) 200ul　363338

特徴

• The enzyme and zymogen forms of digestive enzymes are stored in secretory zymogen granules (ZGs) in the exocrine pancreas.
• Since peptide hormones and cholinergic agents enhance release of ZG contents into the apical medium, ZGs constitute an important storage compartment in the regulated secretory pathway.
• The process of zymogen storage captures large quantities of cellular membranes destined for targeting to the apical plasma membrane
• Fukuoka et al.
• (1991) isolated dog cDNAs encoding GP2, a 75-kD protein that represents the major protein in ZG membranes.
• They found that GP2 is a GPI (glycosylphosphatidylinositol)- linked membrane protein released from the membrane of mature zymogen granules by an enzymatic mechanism.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- F IIRDFLVNVN FQCAYPLDMN VSLQTALQPI VSSLNVDVGG AGEFTVTMAL FQDQSYTHPY EGSKVLLPVE NILYVGALLN RGDTSRFKLL LTNCYATPSG DRNDIVKYFI IRNRCPNQRD STINVEENGV SSESRFSVQM FMFAGNYDLV FLHCEVYLCD STTEQCQPSC STSRLRSSEP AIDLTRVLDI GPITKKSVQN PDTSN
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.