(1996) identified a gene encoding a predicted 158aa protein that is induced by p53.
Because of its homology to glycosylphosphatidylinositol (GPI)-anchored membrane proteins, Furuhata et al.
(1996) designated the gene 'GPI-anchored molecule-like protein,' or GML.
By Northern blot and RT-PCR analysis, Furuhata et al.
(1996) found that GML is expressed as a less than 1-kb mRNA in many tissues, including testis.
Expression of GML suppressed the growth of esophageal cancer cells in culture.
Furuhata et al.
(1996) suggested that GML may play a role in the apoptotic pathway or cell cycle regulation induced by p53 after DNA damage.Kimura et al.
(1997) found that the GML gene consists of 4 exons spanning 12 kb, with a p53-binding site 19 kb upstream of the first exon.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GML.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met21-Pro158 of human GML, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
