GMF-gamma was expressed as a 0.9-kb mRNA predominantly in lung, heart, and placenta.
An additional low-abundance 1.2-kb transcript was detected in heart and skeletal muscle.
Independently, Mao et al.
(1998) identified a cDNA corresponding to GMF-gamma, which they referred to as a GMF-beta homolog, among a collection of cDNAs expressed in hematopoietic stem/progenitor cells
GMF-gamma gene had been mapped to 19q13.2.
This region is frequently deleted in human malignant gliomas and was thus suspected to harbor a glioma tumor suppressor gene.
Given the putative role of GMF-gamma in cell differentiation and proliferation, and its map location, it became a candidate for said tumor suppressor gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GMFg.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Pro12-Asp127 of mouse GMFg, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
