Members of the glucose transporter (GLUT) family, including SLC2A14, are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells.
GLUTs show tissue and cell-type specific expression.
By database analysis, followed by PCR of a human testis cDNA library, Wu and Freeze (2002) cloned SLC2A14.
They identified 2 alternatively spliced forms.
The short isoform has 10 exons and encodes a 497aa protein that shares 94.5% identity with SLC2A3.
The long isoform has 11 exons and encodes a 520aa protein that differs from the short isoform by inclusion of exon 1b.
SLC2A14 contains 12 transmembrane domains, a putative glycosylation site, and sugar transporter signature motifs.
RT-PCR analysis showed that both isoforms were expressed in testis only.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GLUT14.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn51-Val105 of human GLUT14, fused to two N-terminal Tags, His-Tag and MBP-tag, expressed in E. coli.
