The gp160 antigen is a differentiation-related kidney glycoprotein of 160kD that is expressed on the surface of epithelial cells of the glomerulus and proximal tubule cells of the human nephron.
Despite the fact that almost all renal cell carcinomas derive from proximal tubule cells, about 20% of them lack gp160 expression.
Nanus et al.
(1990) showed that expression of gp160 by cultured renal cell carcinomas correlated with the resistance of RCC cells to the antiproliferative effects of alpha-interferon, while lack of expression correlated with the sensitivity to alpha-interferon.
Nanus et al.
(1993) purified the gp160 protein, obtained partial sequences of random peptides, and isolated a full-length cDNA.
The human cDNA shares 78% homology with the murine B-lymphocyte differentiation protein that exhibits aminopeptidase A activity.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GluAP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Gly409-Ala624 of rat GluAP, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
