GA encodes a deduced 602aa protein with a putative N-terminal mitochondrial import presequence.
The GA protein shares 94% and 72% sequence identity with the rat liver and kidney glutaminase proteins, respectively.
Using SDS-PAGE analysis, Gomez-Fabre et al.
(2000) observed a purified fusion GA protein with an apparent molecular mass of 66kD.
Northern blot analysis detected a 2.4-kb GA transcript in human liver, brain, and pancreas, but not in kidney, heart, skeletal muscle, lung, or placenta.
Based on expression data and sequence homology, Gomez-Fabre et al.
(2000) concluded that human GA is most similar to the rat liver-type glutaminase isoform, but is not liver specific as previously predicted from expression data of the rat kidney- and liver-type isoforms.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GLS2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile154-Gly479 of mouse GLS2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
