84-4419-08　GLRX3 (Glutaredoxin 3, TXNL2, PICOT, GRX3, GLRX4, GRX4, Thioredoxin-Like 2, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2) 200ul　363309

特徴

• It has an N-terminal thioredoxin (TXN) homology domain followed by tandem repeats of an 84aa PICOT domain.
• Human PICOT shares 99% aa identity with the rat and mouse orthologs.
• The PICOT domain is highly conserved, and tandem PICOT domains are present in mouse and rat Picot.
• However, only a single PICOT domain is present in nematodes, yeast, bacteria, viruses, and plants.
• Northern blot analysis detected a 1.5-kb PICOT transcript in Jurkat cells.
• RT-PCR detected abundant PICOT expression in heart, spleen, and testis, with lower expression in other tissues.
• Western blot analysis detected PICOT at an apparent molecular mass of 38kD.
• Fractionation of Jurkat cells revealed PICOT in the cytosolic compartment, with very low amounts in the membrane fraction.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EAVPEVSE KYEISSVPTF LFFKNSQKVD RLDGAHAPEL TKKVQRHVSS GAFPPSTNEH LKEDLSLRLK KLTHAAPCML FMKGTPQEPR CGFSKQMVEI LHKHNIQFSS FDIFSDEEVR QGLKTYSNWP TYPQLYVSGE LIGGLDIIKE LEASEELDTI CPKAPKLEER LKVLTNKASV MLFMKGNKQE AKCGFSKQIL EILNSTGVEY ETFDILEDEE VRQGLKTFSN WPTYPQLYVR GDLVGGLDIV
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.