GLI1 encodes a member of the Kruppel family of zinc finger proteins.
The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation.
The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop.
Multiple transcript variants encoding different isoforms have been found for this gene
The zinc fingers contain sequence elements that place the GLI gene product among the Kruppel (Kr) family of zinc finger proteins.
(The German word 'Kruppel' means 'cripple' or 'dwarf.') The Drosophila gene is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GLI1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Val810-Ala1109 of rat GLI1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
