84-4418-99　GLDC (Glycine Dehydrogenase, GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring) 200ul　363301

特徴

• Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase)
• GLDC is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein.
• Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
• The GLDC gene spans at least 135 kb and contains 25 exons.
• All donor and acceptor sites adhered to the canonical GT-AG rule, except for the donor site of intron 21, where a variant form GC is used instead of GT.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TSPFLTHQVF NSYHSETNLV RYMKKLENKD ISLVHSMIPL GSCTMKLNSS SELAPITWRE FANIHPFVPL DQAQGYQQLF QGLEKDLCEI TGYDRVSFQP NSGAQGEYAG LATIRAYLDQ KGERHRTVCL IPKSAHGTNP ASAHMAGMKI QPVEVDRYGN IDVAHLKAMV DQHKENLAAI MITYPSTNGV FEENIGDVCA LIHQHGGQVY LDGANMNAQV GICRPGDFGS DVSHLNLHKT F
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.