Gastrokine-1 is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa.Using differential display of normal and cancerous gastric tissue, followed by 5-prime RACE, Yoshikawa et al.
(2000) identified cDNAs encoding CA11 and CAPN9
The deduced 199aa CA11 protein contains an asn-rich region.
Northern blot analysis revealed expression of an approximately 1.1-kb CA11 transcript predominantly in stomach; no expression was detected in cancer tissue or gastric cancer cell lines.
Yoshikawa et al.
(2000) identified a marker in the CA11 gene that indicates a localization on chromosome 2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GKN1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala22-Asn199 of human GKN1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
