84-4418-91　［受注停止］GIF (Gastric Intrinsic Factor, TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis) 200ul　363293

特徴

• Intrinsic factor is a glycoprotein produced by the parietal cells of the stomach.
• It is necessary for the absorption of vitamin B12 later on in the terminal ileum
• Upon entry into the stomach, vitamin B12 becomes bound to haptocorrin (R factor), a glycoprotein.
• The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin.
• In the less acidic environment of the small intestine, B12 can then bind to intrinsic factor.
• This new complex travels to the ileum, where special epithelial cells endocytose them.
• Inside the cell, B12 dissociate once again and bind to another protein, transcobalamin II.
• The new complex can exit the epithelial cells to enter the liver.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LFG QVLKDIVEKI SMKIKDNGII GDIYSTGLAM QALSVTPEPS KKEWNCKKTT DMILNEIKQG KFHNPMSIAQ ILPSLKGKTY LDVPQVTCSP DHEVQPTLPS NPGPGPTSAS NITVIYTINN QLRGVELLFN ETINVSVKSG SVLLVVLEEA QRKNPMFKFE TTMTSWGLVV SSINNIAENV NHKTYWQFLS GVTPLNEGVA DYIPFNHEHI TAN
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.