Intrinsic factor is a glycoprotein produced by the parietal cells of the stomach.
It is necessary for the absorption of vitamin B12 later on in the terminal ileum
Upon entry into the stomach, vitamin B12 becomes bound to haptocorrin (R factor), a glycoprotein.
The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin.
In the less acidic environment of the small intestine, B12 can then bind to intrinsic factor.
This new complex travels to the ileum, where special epithelial cells endocytose them.
Inside the cell, B12 dissociate once again and bind to another protein, transcobalamin II.
The new complex can exit the epithelial cells to enter the liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GIF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Asp31-Cys288 of mouse GIF, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
