GGH catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma- glutamates by the removal of gamma-linked polyglutamates and glutamate.
The cDNA encodes a 318aa protein with a deduced amino acid sequence 67% identical to that of the rat enzyme.
The N-terminal 24 residues are likely a leader sequence that mediates translocation of GGH into the endoplasmic reticulum for secretion.
GGH also contains 4 potential N-glycosylation sites.
Western blot analysis detected GGH at an apparent molecular mass of about 35kD.
Human GGH showed higher activity toward the pentaglutamate derivative of methotrexate and had little activity against the diglutamate derivative.
More than 60% of the total GGH activity was secreted into the medium of the 5 tumor cell lines examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human gGH.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg25-Asp318 of human gGH, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
