GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve.
RET stimulation in trans by soluble or immobilized GFRA1 potentiates downstream signaling, neurite outgrowth, and neuronal survival, and elicits dramatic localized expansions of axons and growth cones
Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.
Activated RET associates with different adaptor proteins inside and outside lipid rafts.
The authors concluded that these results provide an explanation of the tissue distribution of GFRA1, supporting the physiologic importance of RET activation in trans as a mechanism to potentiate and diversify the biologic responses to GDNF.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GFRa1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu239-Asp350 of mouse GFRa1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
