Glutamine:fructose-6-phosphate amidotransferase (GFAT) is the first and rate-limiting enzyme of the hexosamine biosynthesis pathway.
The enzyme transfers the amide group from L-glutamine to fructose 6-phosphate to form glucosamine 6-phosphate.
See GFAT1 (GFPT1).
By searching an EST database for sequences related to GFAT1, Oki et al.
(1999) identified a cDNA encoding a new GFAT subtype that they called GFAT2.
The predicted 682aa human protein shares 76% and 97% similarity to human GFAT1 and mouse Gfat2, respectively.
Recombinant GFAT2 exhibited GFAT enzyme activity in vitro.
Northern blot analysis revealed that GFAT2 was expressed predominantly as a 3-kb mRNA, although additional transcripts were present in some tissues.
The highest levels of expression were observed in heart, placenta, and spinal cord.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GFPT2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys2-Gly288 of human GFPT2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
