Protease nexin I is a 44kD thrombin and urokinase inhibitor released by human foreskin fibroblasts.
PN-I shares several features with antithrombin III, an abundant plasma thrombin inhibitor.
Both PN-I and AT-III have high affinities for heparin, and heparin accelerates their rate of thrombin inhibition.
In addition, the published sequence of 28aa at the N-terminus of PN-I is identical to the N-terminal amino acid sequence of a glial-derived neurite promoting factor.
Two forms, designated alpha and beta, were identified.
These differed only by the insertion of 3 nucleotides into the coding sequence, and apparently arose by utilization of an alternative splice acceptor site in the PN1 gene.
In the resulting proteins, alpha-PN-I contains an arginine residue at position 310, whereas beta-PN-I contains threonine-lysine residues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GDN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser20-Pro398 of human GDN, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
