Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function.
Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
GDF9 is a member of the transforming growth factor-beta (TGF-Beta) superfamily.
GDF9 plays an important role in the development of primary follicles in the ovary.
It has a critical role in granulosa cell and theca cell growth, as well as in differentiation and maturation of the oocyte.GDF9 has been connected to differences in ovulation rate and in premature cessation of ovary function, therefore has a significant role in fertility.
The cell surface receptor through which GDF9 generates a signal is the bone morphogenetic protein type II receptor (BMPR2
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GDF9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Arg306-Arg440 of rat GDF9, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
