Guanine deaminase (GDA) catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.Cypin localizes to both pre- and postsynaptic sites, is enriched in dendritic shafts, cofractionates, and associates with membrane-associated guanylate kinase proteins (PSD95/93 and SAP97/102) in the synaptic plasma membranes and vesicles.
Cypin also occurs in the epithelial cells of the small intestine, highest at the tips of the intestinal villi, and exclusively at the basal membrane.
GST fusion experiments showed that cypin binding to PSD95 occurs through a C-terminal consensus sequence and requires PDZ domains 1 and 2.
Overexpression of cypin in rat hippocampal neurons specifically perturbs postsynaptic trafficking of PSD95 and SAP102, an effect not produced by overexpression of other PDZ ligands.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GDA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Val454 of human GDA, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
