G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage.
It is produced by activated monocytes/macrophages, fibroblasts, endothelial cells, astrocytes, osteoblasts and bone marrow stromal cells.
In addition, various carcinoma cell lines and myeloblastic leukemia cells can produce G-CSF constitutively.
G-CSF and G-CSF receptors have also been shown to be produced by placental, decidual and endometrial gland cells during pregnancy, suggesting that G-CSF may play a role in decidual and placental functions.
Clinical use of G-CSF has been approved for the amelioration of chemotherapy-induced neutropenia as well as for severe chronic neutropenia following bone marrow transplants.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes bovine GCSF.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to Cys38-Pro195 of bovine GCSF, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
