Guanylate-binding proteins, such as GBP4, are induced by interferon and hydrolyze GTP to both GDP and GMP.Vestal (2005) reported that the 642aa GBP4 protein shares 50 to 55% identity with GBP1 , GBP2 , and GBP5 .
GBP4 contains a GTP-binding domain, but it lacks a C-terminal CaaX isoprenylation motif found in the other 3 GBPs.
Olszewski et al.
(2006) found that GBP4 shares 74% and 81% aa identity with GBP6 and GBP7 , respectively.
All GBPs, including GBP4, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases.
GBP4 has a unique 15aa group at its N terminus that is not found in other GBPs.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GBP4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Cys304 of mouse GBP4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
