Growth arrest-specific 6 is similar to the Protein S with the same domain organization and 43% amino acid identity.
GAS6 is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation.
The gene is expressed as an abundant 2.6-kb mRNA in starved cells but decreases significantly upon stimulation of cells with serum or fibroblast growth factor.
A human homolog was isolated from HeLa cells and was predicted to encode a 678aa protein with 81% identity to the mouse protein and 44% identity to human protein S, a vitamin K-dependent negative coregulator in the blood coagulation pathway.
Regions of greatest similarity between Gas6 and protein S occur in a gamma carboxylated glutamic acid-rich domain and in 4 EGF-like domains.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GAS6.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala483-Gly646 of mouse GAS6, fused to N-terminal His-Tag expressed in E. coli.
