Guanidinoacetate N-methyltransferase is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor.
Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals.
Two transcript variants encoding different isoforms have been described for this gene
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases.
The systematic name of this enzyme class is S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase.
This enzyme participates in glycine, serine and threonine metabolism and arginine and proline metabolism.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GAMT.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser2-His236 of mouse GAMT, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
