Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain.
The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical.
Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different.
Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands.
Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized.
During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes porcine GAL4.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Po
Immunogen：Recombinant protein corresponding to Met1-Ile323 of porcine GAL4, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
