The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion.Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins.
Galectin-2, a lectin binding to beta-galactoside, was found to trigger apoptosis of activated T cells and leaves naive or memory T cells unaltered.
Cytokine secretion of activated T cells was significantly shifted to the Th2 profile.
In vivo experiments showed a significant decrease of bowel inflammation during the course of several days in galectin-2 treated mice featuring DSS-colitis.
Furthermore, contact hypersensitivity (CHS) responses were reduced significantly in Galectin-2 treated mice after sensitization to contact allergens.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA:1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GAL2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat GAL2, Met1-Glu130, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
