GADD45a is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents.
The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase.
The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
GADD45 is the only known x-ray responsive gene whose induction is not mediated by PKC.
Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165aa polypeptide that is 96% identical in the 2 species
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat GADD45a.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Met1-Arg165 of rat GADD45a, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
