Glutamate decarboxylase 1 (brain, 67kD) is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes.
The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid.
A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes.
This gene may also play a role in the stiff man syndrome.
Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.
Alternative splicing of this gene results in two products, the predominant 67kD form and a less-frequent 25kD form.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GAD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Leu97 of mouse GAD1, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
