GABARAPL2 contains 117aa and has a calculated molecular mass of 13.7kD.
Like GABARAP, it contains a basic N-terminal tubulin binding domain and a GABA-A receptor binding domain.
GABARAPL2 shares 57% identity with GABARAP and 100% amino acid sequence identity with bovine brain GATE16, rat Gabarapl2, and mouse Gabarapl2.
Northern blot analysis revealed ubiquitous expression of a 1.35-kb transcript with high levels in heart, brain, testis, prostate, ovary, spleen, and skeletal muscle, and low levels in lung, thymus, and small intestine.
GABARAPL2 had activity as a soluble transport factor, it interacted with N-ethylmaleimide- sensitive factor (NSF) and stimulated its ATPase activity, and interacted with the Golgi v-SNARE (GOS28) in an NSF-dependent manner.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GABARAPL2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Phe117 of human GABARAPL2, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
