GAB3 is a member of the GRB2-associated binding protein gene family.
These proteins are scaffolding/docking proteins that are involved in several growth factor and cytokine signaling pathways, and they contain a pleckstrin homology domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein.
The protein encoded by this gene facilitates macrophage differentiation.
Two transcript variants encoding different isoforms have been found for this gene.
The deduced 586aa protein contains an N-terminal pleckstrin homology (PH) domain and potential SH2- and SH3-binding motifs.
The PH domains of GAB1, GAB2, and GAB3 share about 50% amino acid identity, while the remainder of the proteins share about 14% identity.
Gab3 was expressed in all myeloid and macrophage cell lines examined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse GAB3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Pro142-Asn375 of mouse GAB3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
