Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family.
It may have a role in leukemogenesis.
The FSTL3 cDNA, which they called FLRG (follistatin-related gene), encodes a deduced 263aa protein that shares 85% amino acid identity with mouse Fstl3.
It contains 2 cysteine-rich repeats that are closely related to the class 1-1 follistatin modules present in other follistatin family members.
Northern blot analysis demonstrated expression of a 2.5-kb transcript in placenta, amniocytes, chorionic villosities, and bone marrow stromal cells, with weaker expression in fibroblasts, keratinocytes, and endothelial cells.
A 1.3-kb transcript was also detected in some human tissues and was thought to result from use of an alternate polyadenylation site.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FSTL3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Val24-Val256 of mouse FSTL3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
