The deduced 508aa protein has a predicted molecular mass of 56.8kD.
It contains a myristylation sequence, a phosphotyrosine-binding (PTB) domain, and 4 potential GRB2-binding sites.
Northern blot analysis of adult mouse tissues detected ubiquitous expression of Frs2, with highest levels in brain, kidney, lung, ovary, and testis.
SDS-PAGE showed that FRS2 migrates as doublet of 92 to 95 kD
Melillo et al.
(2001) presented evidence that FRS2 couples both ligand-regulated and oncogenic forms of RET with the MAPK signaling cascade under normal biologic conditions and pathologic conditions, such as multiple endocrine neoplasias and papillary thyroid carcinomas.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FRS2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu268-Pro453 of mouse FRS2, expressed in E. coli.
