Fyn-related kinase is an enzyme belongs to the TYR family of protein kinases.
This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth.Sequence analysis predicted that the 505aa FRK protein is 49% and 47% identical to FYN and CSK, respectively.
FRK contains a putative ATP-binding motif and tyrosine kinase signature motifs, as well as SH2 and SH3 domains; however, it lacks a myristylation signal found in FYN.
Northern blot analysis revealed expression of a 3.0-kb transcript in a hepatoma cell line, but expression in other cell lines or normal tissues was not detected.
PCR amplification detected expression in some cell lines.
Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FRK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Val234-Phe49 of human FRK, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
