Fibrinopeptide A is a substance released as part of the blood clotting process.
Fibrinogen, the principal protein of vertebrate blood clotting is a hexamer containing two sets of three different chains (alpha, Beta, and gamma), linked to each other by disulfide bonds.
The N-terminal sections of these three chains contain the cysteines that participate in the cross-linking of the chains.
The C-terminal parts of the alpha, Beta and gamma chains contain a domain of about 225aa residues, which can function as a molecular recognition unit.
In fibrinogen as well as in angiopoietin this domain is implicated in protein-protein interactions.
In lectins, such as mammalian ficolins and invertebrate tachylectin 5A, the fibrinogen C-terminal domain binds carbohydrates.
On the fibrinogen alpha and Beta chains, there is a small peptide sequence (called a fibrinopeptide).
It is these small peptides that prevent fibrinogen spontaneously forming polymers with itself.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
AA Sequence:ADTGTTSEFIEAGGDIR
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FPA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human FPA, conjugated to OVA.
