The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2.
These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1
Structure-function analysis demonstrated that the major carboxy-terminal transactivation domains of Fos and FosB are dispensable and that Fosl1, which lacks transactivation domains, has the highest rescue activity.
Moreover, a transgene expressing Fosl1 rescued the osteopetrosis of Fos mutant mice in vivo.
The osteoclast differentiation factor Rankl induced transcription of Fosl1 in a Fos-dependent manner, thereby establishing a link between Rank signaling and the expression of Ap1 proteins in osteoclast differentiation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse FOSL1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Leu273 of mouse FOSL1, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
