FN3K catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines followed by Amadori rearrangement.
Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins.FN3K is responsible for the formation of fructose 3-phosphate (F3P), a compound identified in the lenses of diabetic rats.
The spontaneous decomposition of F3P leads to the formation of 3-deoxyglucosone (3DG).
3DG contributes to diabetic complications.
Treatment of normal and diabetic rats with an inhibitor of FN3K demonstrated a large reduction (~50%) in systemic 3DG in both groups.
Removal of 3DG at its source by inhibition of FN3K is a viable option to treat diabetes related diseases since it would require a much smaller dose of drug.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FN3K.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Lys309 of human FN3K, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
