FLRT3 shares 55% amino acid sequence identity with FLRT1 and 44% identity with FLRT2.
FLRT3 is expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart.
Two alternatively spliced transcript variants encoding the same protein have been described for this gene
The predicted 649aa FLRT3 protein contains a putative N-terminal signal sequence, 10 leucine-rich repeats (LRRs) flanked by N- and C-terminal cysteine-rich regions, a fibronectin-/collagen-like domain, a transmembrane domain, and an intracellular C-terminal tail.
FLRT3 has 4 potential N-glycosylation sites in its extracellular region.
FLRT3 shares 55% amino acid sequence identity with FLRT1 and 44% identity with FLRT2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human FLRT3.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Lys29-Asp152 of human FLRT3, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.