FLII encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain.
The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle.
The gene is located within the Smith-Magenis syndrome region on chromosome 17
The amino acid sequence deduced from the FLI cDNA has 52% similarity to that of the human gelsolin protein and also has an N-terminal leucine-rich domain with 16 consecutive leucine-rich repeats.
FLII gene encodes a predicted 1269aa protein.
Northern blot analysis revealed FLII expression as an approximately 4.4-kb mRNA in all tissues tested, with strongest expression in skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FLII.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu896-Phe1176 of human FLII, fused to two N-terminal Tags, His-Tag and GST-tag, expressed in E. coli.
